Production of Indole-3-Acetic Acid by Bacillus Isolated from Different Soils

A. F. CHAGAS JUNIOR1, A. G. de OLIVEIRA1, L. A. de OLIVEIRA2, G. R. dos SANTOS1, L. F. B. CHAGAS1, A. L. Lopes da SILVA3 and J. da LUZ COSTA1,3
1 Federal University of Tocantins, Department of Agricultural Science and Technology, 77402-970 Gurupi – TO, Brazil
2 Instituto Nacional de Pesquisas da Amazônia INPA/CPCA, 69011-970 Manaus – AM, Brasil
3 Federal University of Parana, Department of Bioprocess Engineering and Biotechnology, 81531-970, Curitiba – PR, Brazil

Abstract

CHAGAS JUNIOR, A. F., A. G. de OLIVEIRA, L. A. de OLIVEIRA, G. R. dos SANTOS, L. F. B. CHAGAS, A. L. Lopes da SILVA and J. da LUZ COSTA, 2015. Production of indole-3-acetic acid by Bacillus isolated from different soils. Bulg. J. Agric. Sci., 21: 282–287

Bacillus sp. can be found in different environments, produce endospores and being known as Plant Growth Promoting. The aim of this study was to evaluate the ability of Bacillus thuringiensis and Bacillus cereus isolated from soil samples from various regions in the State of Tocantins, in the production of auxin. Both in the absence and in the presence of L-tryptophan all bacteria tested produced auxin. The production of auxin increased with the increasing of the concentrations of L-tryptophan, especially the isolates UFT Bt 07, UFT Bc 19 and UFT Bc 20, which presented the highest levels of production. Twenty isolates, 12 (60%) had concentrations of auxin more than 100 μg mL-1. For most of the isolates the increased production of auxin occurred with 48 hours of bacterial growth. Bacilli tested have great ability to biosynthesize auxin from tryptophan as a precursor; can be considered as probable growth promoters of plant.

Key words: Plant growth promoting rhizobacteria (PGPR), phytohormones, Soil biology, indole-3-acetic acid, L-tryptophan

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