M. BONOVSKA, K. GURGULOVA, S. TAKOVA and T. SAVOVA
National Diagnostic and Research Veterinary Medical Institute “Proof. Dr. G. Pavlov”, BG - 1606 Sofia, Bulgaria
BONOVSKA, M., K. GURGULOVA, S. TAKOVA and T. SAVOVA, 2014. Molecular diagnosis of American foulbrood in honeybee brood in Bulgaria. Bulg. J. Agric. Sci., 20: 899-902
The analysis of the 47 isolates for identification of Paenibacillus larvae, obtained of honeybee brood with American foulbrood (AFB) symptoms from 9 different districts of Bulgaria by conventional PCR technique was performed. Specific DNA products by size 1106 bp were obtained from the 42 isolates. Five isolates were PCR negative for AFB (P. larvae). The results showed that PCR assay is specific and sensitive method for molecular identification of Paenibacillus larvae and can provide a rapid and reliable diagnosis of infected honeybee brood.