BYMV on Chickpea Germplasm in Bulgaria

S. PETROVA1, G. PASEV2 and D. KOSTOVA2
1 Institute of Plant Genetic Resources, BG – 4202 Sadovo, Bulgaria
2 Maritsa Vegetable Crops Research Institute, BG – 4002 Plovdiv, Bulgaria

Abstract

PETROVA, S., G. PASEV and D. KOSTOVA, 2014. BYMV on Chickpea Germplasm in Bulgaria. Bulg. J. Agric.Sci., 20: 150-154

 

During May and June (2010 and 2011) virus symptoms were observed on significant part of chickpea germplasm grown in native plant nursery of the Institute of Plant Genetic Resources, Sadovo. The symptoms consisted of severe narrowing and twisting of the upper leaflets up to filiform leaves and redness of older leaves. Bean yellow mosaic virus (BYMV ) was identified in all tested samples using test-plants, ELISA and RT-PCR. Infectious tests with BYMV isolate were carried out in order to evaluate the level of susceptibility of chickpea germplasm. Most of the tested accessions and varieties appeared to be virus susceptible. Few were selected as virus tolerant. Further investigations on progenies are necessary in order to confirm the genetic control of the observed level of resistance.

Key words: Cicer arietinum L., Potyvirus, ELISA, RT-PCR, virus susceptibility, virus tolerance

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