Effect of Plant Growth Regulators on Chloroplast Ultra Structure in Lamium Album Plantlets

Sofia University “St. Kliment Ohridski”, Department of Botany, Faculty of Biology, BG - 1164 Sofia, Bulgaria


STEFANOVA, M., D. KOLEVA and T. GANEVA, 2013. Effect of plant growth regulators on chloroplast ultra structure in Lamium album plantlets. Bulg. J. Agric. Sci., 19: 1208-1212


Lamium album L. stem nodal segments were grown on Murashige and Skoog’s medium without growth regulators (control) and on media supplemented with 0.2 mg l-1 N6-benzyladenine, 0.5 mg l-1 indole-3-butyric acid or both plant growth regulators. The plastid apparatus of in vitro formed leaflets was studied by transmission electron microscopy. The cytokinin N6-benzyladenine negatively affected the chloroplast formation when was applied individually or in combination with the auxin indole-3-butyric acid. On the contrary, IBA in concentration of 0.5 mg l-1 was appropriate for development of chloroplasts with regular shape and well organized internal membrane system thus providing for the regeneration capacity in vitro.

Key words: in vitro-regeneration, indole-3-butyric acid, medicinal plant, N6-benzyladenine, transmission electron microscopy, white dead nettle
Abbreviations: BA - N6-benzyladenine; IBA - indole-3-butiric acid; PGRs - plant growth regulators; TE M - transmission electron microscopy

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