B. NEOV1, D. TEOFANOVA1, L. ZAGORCHEV2, G. RADOSLAVOV1 and P. HRISTOV1
1 Bulgarian Academy of Sciences Institute of Biodiversity and Ecosystem Research, BG – 1113 Sofia, Bulgaria
2 Sofia University “St. Kliment Ohridski”, Faculty of Biology, BG – 1164 Sofia, Bulgaria
Neov, B., D. Teofanova, L. Zagorchev, G. Radoslavov and P. Hristov, 2013. Milk protein polymorphism in Bulgarian grey cattle population. Bulg. J. Agric. Sci., Supplement 2, 19: 194–196
Application of gene specific molecular markers in genotyping and genetic identification is of an essential significance for preserving genetic diversity. The aim of the present research is to reveal the genotype profile of native Bulgarian Grey cattle population with respect to lactoprotein genes: kappa-casein and αS1-casein through PCR-RFLP analysis. The results for kappa-casein locus show superiority of heterozygous AB genotype and prevalence of the uncommon B allele. Analysis of the polymorphism of alfaS1-casein gene displays high frequency of heterozygous BC genotype and C allele of gene. Genetic profile of the breed is compared to other cattle populations to establish the position of the breed about other European cattle breeds genetic diversity. It may be concluded that Bulgarian grey cow is with specific genotype profile similar to other cattle population in South-Eastern Europe.