Comparative Anti-Herpes Effects of the Chloroform in vitro and in vivo Extracts, Derived from Lamium Album L.

D. TODOROV1, M. DIMITROVA2, K. SHISHKOVA1, ZH. YORDANOVA2, V. KAPCHINA-TOTEVA2 and S. SHISHKOV1
1  Sofia University “St. Kl. Ohridski”, Laboratory of Virology, BG – 1164 Sofia, Bulgaria
2 Sofia University “St. Kl. Ohridski”, Department of Plant Physiology, Faculty of Biology, BG – 1164 Sofia, Bulgaria

Abstract

Todorov, D., M. Dimitrova, K. Shishkova, Zh. Yordanova, V. Kapchina-Toteva  and S. Shishkov, 2013. Comparative anti-herpes effects of the chloroform in vitro and in vivo extracts, derived from Lamium album l. Bulg. J. Agric. Sci., Supplement 2, 19: 190–193

 

Lamium album L. is a valuable medicinal plant which possesses astringent, spasmolytic, anti-inflammatory, antibiotic and bacteriostatic properties. In our study, chloroform extracts derived by Soxhlet extraction from in vivo and in vitro propagated plants were tested for antiviral activity. The extracts inhibited significantly the replication of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in MDBK cells, without apparent cytotoxicity. The extracts showed strong antiviral effect. The 50% inhibitory concentrations (IC50) of the chloroform in vitro extract (CS) were 552μg.mL-1 and 487 μg.mL-1, respectively. The IC50 of the chloroform in vivo extract (CES) were 668μg.mL-1 and 780 μg.mL-1, respectively. Viral replication was suppressed over 90% when both chloroform extracts were applied at maximal tolerated concentrations (MTC). The data suggested that extracts derived from wild plants have stronger inhibitory effects than in vitro extracts. The chloroform in vivo extract CES showed strong virucidal effect. The extract applied in MTC inactivated the extracellular HSV-1 after 5 minutes of contact at a rate exceeding 90% (∆log1.5).

Key words: in vitro and in vivo extracts, Lamium album, herpes simplex virus, viral inhibitor, virucidal effect
Abbreviations: CS – chloroform in vitro extract, CES – chloroform in vivo extract

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