Influence of Complement Activation on Established Zymosan-Induced Arthritis

L. BELENSKA1, P. DIMITROVA2 and N. IVANOVSKA2
1 Sofia University “St. Kliment Ohridsky”, Department of Biology, Medical Genetics and Microbiology, Faculty of Medicine, BG – 1164 Sofia, Bulgaria
2 Bulgarian Academy of Sciences, Department of Immunology, Institute of Microbiology, BG – 1113 Sofia, Bulgaria

Abstract

BELENSKA, L., P. DIMITROVA and N. IVANOVSKA, 2013. Influence of complement activation on established zymosan-induced arthritis. Bulg. J. Agric. Sci., Supplement 2, 19: 163–166

 

Zymosan-induced arthritis is an experimental model of rheumatoid arthritis used for analyzing cells and molecules that mediate the pathogenesis of inflammatory joint diseases. Although, the alternative pathway of complement is involved in the pathogenesis of arthritis much issues are not well elucidated. The aim of the present study was to evaluate the effect of complement depletion on cell populations that participate in the course of zymosan-induced arthritis. The decomplementation was performed using cobra venom factor (CVF), a peptide fragment of cobra C3 component, which is capable of activating the alternative complement pathway. We established that CVF treatment affected proteoglycan loss and collagen synthesis dependent on time of injection. Absence of complement activity differentially affected CD49+ and CD49/CD94+ cell populations in the synovium and blood. In addition, the influx of synovial CD4+ T cells is ameliorated in CVF-treated mice, compared to untreated arthritis mice. This positive effect achieved by complement inhibition in our experimental model is related to local and systemic changes in cell populations.

Key words: complement, rheumatoid arthritis, zymosan-induced arthritis

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