Changes in the Actin Cytoskeleton and Actin-Associated Proteins in Hela Cells as a Result of E. Coli O157:Н7 Infection

T. TOPOUZOVA-HRISTOVA1, S. STOITSOVA2, T. PAUNOVA-KRASTEVA2, J. DOUMANOV3 and E. STEPHANOVA1
1 Sofia University”St. Kliment Ohridski”, Department of Cytology, Histology and Embryology, Faculty of Biology, BG – 1164 Sofia, Bulgaria
2 Bulgarian Academy of Sciences, Institute of Microbiology, BG – 1113 Sofia, Bulgaria
3 Sofia University”St. Kliment Ohridski”, Department of Biochemistry, Faculty of Biology, BG – 1164 Sofia, Bulgaria

Abstract

TOPOUZOVA-HRISTOVA, T., S. STOITSOVA, T. PAUNOVA-KRASTEVA, J. DOUMANOV and E. STEPHANOVA, 2013. Changes in the actin cytoskeleton and actin-associated proteins in HeLa cells as a result of E. coli O157:Н7 infection. Bulg. J. Agric. Sci., Supplement 2, 19: 155–158

 

Escherichia coli strains with the O157 LPS serotype may cause health disorders with variable severity. These bacteria are extracellular pathogens, which have developed a specific mode to manipulate host cell cytoskeleton by means of a Type III secretion system. On the background of the very well analyzed F-actin rearrangements, little is known about the possible interference of E. coli O157 adherence with other cytoskeleton-related proteins and events in non-polarized host cells. Here we describe data indicating alterations of the actin-associated proteins ZO-1 and villin during co-incubation of HeLa cells with a strain of E. coli O157:H7. Using laser scanning confocal microscopy our data demonstrate unusual cytoskeletal actin rearrangement in HeLa cell line comparing to polarized epithelial cells. Additionally, behavior of actin-associated proteins ZO-1 and villin, as well as lack of F-actin patches, suggested a different manner of bacterial pathogenesis in non-polarized epithelial cells.

Key words: EHEC, A/E lesions, villin, ZO-1
Abbreviations: CM-PBS – phosphate buffered saline with Ca and Mg; FAS – fluorescent-actin staining; LA – localized adhesion; LAL – localized adhesion like; EHEC – enterohaemorrhagic E. coli; FITC – fluorescein isothiocyanate; BSA – bovine serum albumin, NCIPD – National Center of Infectious and Parasitic Diseases; A/E attaching-effacing

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