Ammonium Vanadate Decreases Viability and Proliferation of Cultured Retrovirus-Transformed Chicken Hepatoma Cells

1 Sofia University “St. Kliment Ohridski”, Faculty of Biology BG – 1164 Sofia, Bulgaria
2 Bulgarian Academy of Sciences, Institute of Experimental Morphology, Pathology and Anthropology with Museum, BG – 1113 Sofia, Bulgaria
3 Bulgarian Academy of Sciences, Institute of Neurobiology, BG – 1113 Sofia, Bulgaria


Abudalleh, A. M., T. Zhivkova, L. Dyakova, B. Andonova-Lilova and  R. Alexandrova, 2013. Ammonium vanadate decreases viability and proliferation of cultured retrovirus-transformed chicken hepatoma cells. Bulg. J. Agric. Sci., Supplement 2, 19: 151–154


Vanadium compounds have been reported to possess anti-neoplastic potential especially against chemically induced liver, mammary, and colon carcinogenesis in rodents.  According to the literature available, the anticancer activity of vanadium compounds in virus-transformed tumor cells has not been clarified yet. The aim of the study presented was to evaluate the effect of ammonium vanadate (NH4VO3) on viability and proliferation of cultured LSCC-SF-Mc29 cell line established from a transplantable chicken hepatoma, induced by the myelocytomatosis virus Mc29. The investigations were performed by thiazolyl blue tetrazolium bromide (MTT) test, neutral red uptake cytotoxicity assay (NR), colony-forming method (CFM) and double staining with acridine orange and propidium iodide (PI/AO). The results obtained revealed that applied at a concentration range of 0.1–20  µg/ml   for 24–72 h NH4VO3 expresses significant cytotoxic and/or cytostatic effects that are time- and concentration dependent. Administered at concentrations ≥ 5 µg/ml this compound completely inhibit the colony-forming ability of chicken hepatoma cells. 

Key words: ammonium vanadate, cell culture, chicken hepatoma, cytostatic/cytotoxic activity, myelocytomatosis virus

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