Electrokinetic Potential of Pea Thylakoid Membranes under Static Magnetic Field (2t) Treatment: Role of Iron Ions and Light Exposure

E. IVANOVA1,4, V. DOLTCHINKOVA2 and CH. ROUMENIN3
1 Bulgarian Academy of Sciences, Institute for Molecular Biology “Acad Roumen Tsanev”, Department of Gene Regulations, BG – 1113 Sofia, Bulgaria
2 Sofia University “St. Kliment Ohridski”, Department of Biophysics and Radiobiology; Faculty of Biology, BG – 1164 Sofia, Bulgaria
3 Bulgarian Academy of Sciences, Institute of Systems Engineering and Robotics (ISER), BG – 1113 Sofia, Bulgaria
4 Katholieke Universiteit Leuven, Laboratory of Protein Phosphorylation, 3000 Leuven, Belgium

Abstract

Ivanova , E., V. Doltchinkova and Ch. Roumenin, 2013. Electrokinetic potential of pea thylakoid membranes under static magnetic field (2t) treatment: role of iron ions and light exposure. Bulg. J. Agric. Sci., Supplement 2, 19: 147–150

 

The effects of static magnetic field (SMF, 2T) on pea thylakoid membranes were investigated under photosynthetic and actinic light exposure. The pea plants were subjected to light intensity of 2000 lx (HL, high light) and to 650 lx (IL, intermediate light), respectively in order to specify the effect of SMF (2T) on the electrokinetic potentials at the membrane surfaces. We determined the electrophoretic mobility, zeta potential and surface charge density of thylakoids in dependence on the iron content of the ‘Fe-sufficient’, ‘Fe-starved’ and ‘2 x Fe’ variants. The generation of light-induced increase in negative zeta potential of ‘Fe-sufficient’ SMF (2T) pre-exposed thylakoids (HL, IL) was due to an enhancement of net negative surface electric charge on the outer surface of the membranes.
The primary electron-transport processes across the thylakoid membrane was increased due to actinic light treatment to the ‘Fe-starved’ SMF pre-exposed thylakoids (HL) because of most densely packed thylakoids.
A decreased lipid peroxidation by SMF (2T) exposure before or after actinic illumination of thylakoids was observed. It could be due to a recombination of radical products as a consequence of an indirect action of magnetic field on thylakoids from ‘Fe-sufficient’ and ‘2 x Fe’  plants.
The electrokinetic potential changes in pea thylakoids upon SMF (2T) exposure depend on Fe content in growing medium as well as light intensity (HL, high light; IL, intermediate light) during pea plant growing.

Key words: Surface charge density; Static magnetic field; Light scattering; Thylakoid membranes; Lipid peroxidation

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