New Hybrid Matrix for Immobilization of Trichoderma Viride SL-45

Y. EVSTATIEVA1, M. BADALOVA1, G. CHERNEV2, D. NIKOLOVA1 and V. SAVOV1
1 Sofia University “St. Kliment Ohridski”, Department of Biotechnology, Faculty of Biology, BG – 1164 Sofia, Bulgaria
2 UCTM, Department of Silicate Technology, Faculty of Metallurgy and Material Sciences, BG – 0000 Sofia, Bulgaria

Abstract

Evstatieva, Y., M. Badalova, G. Chernev, D. Nikolova and V. Savov, 2013. New hybrid matrix for immobilization of Trichoderma viride SL-45. Bulg. J. Agric. Sci., Supplement 2, 19: 120–122

 

In this work Trichoderma viride SL-45, was investigated in new a hybrid matrix by immobilizing method. Vegetative culture of this strain was immobilized in four variations of sol-gel hybrid silica materials, composed of tetraethylorthosilicate as an inorganic precursor, 10 % polyethylene oxide and 5, or 10% carboxymethylcellulose as organic compounds. It was found that the new hybrid matrix of immobilization had positive effect on cellulase activity of strain. Biosynthetic activity of immobilized cultures was investigated by batch cultivation up to 840th h. The production of cellulase from immobilized culture in hybrid matrix composed of tetraethylorthosilicate (as precursor) and 5% carboxymethylcellulose (as organic compound and substrate) was found to be about twice higher than the activity of free Trichoderma viride SL-45 culture.

Key words: immobilization; hybrid matrix, Trichoderma; cellulase activity

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