Enhancement of Xylanase Production by Sol-Gel Immobilization of Aspergillus Awamori K-1

M. YORDANOVA1, Y. EVSTATIEVA1, G. CHERNEV2, S. ILIEVA1, R. DENKOVA1 and D. NIKOLOVA1
1 Sofia University “St. Kliment Ohridski”, Department of Biotechnology, Faculty of Biology, Sofia, Bulgaria
2 UCTM, Department of Silicate Technology, Faculty of Metallurgy and Material Sciences, Sofia, Bulgaria

Abstract

Yordanova, M., Y. Evstatieva, G. Chernev, S. Ilieva, R. Denkova and D. Nikolova, 2013. Enhancement of xylanase production by sol-gel immobilization of Aspergillus awamori K-1. Bulg. J. Agric. Sci., Supplement 2, 19: 117–119

 

Technological potential of Aspergillus awamori species is of particular interest in the investigation of new methods for increasing the cell viability and xylanase activity. The purpose of the present investigation was to study the immobilization of Aspergillus awamori K-1cells for higher xylanase production using sol-gel encapsulation in hybrid matrices composed of tetraethylorthosilicate as an inorganic precursor, 5% xylan and 5, 10 or 15% calcium alginate as organic compounds. The enzyme synthesis of immobilized cells under batch fermentation conditions were compared with xylanase activity of free cell culture used as control. According to the results, culture immobilized in hybrid matrix composed of tetraethylorthosilicate and 5% calcium alginate was found to display xylanase activity of 28,88 IU.ml-1 higher than the control enzyme activity of 22,04 IU.ml-1. In contrast to the free cell culture, immobilized cells retain their viability and biosynthetic capabilities during continuous fermentation processes.

Key words: Aspergillus, xylanase, immobilization, sol-gel hybrids, alginate

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