Identification of Xanthomonas Strains from Tomato and Pepper and Their Sensitivity to Antibiotics and Copper

Y. KIZHEVA1, T. VANCHEVA1, P. HRISTOVA1, M. STOYANOVA2, M. STOJANOVSKA1, P. MONCHEVA1 and N. BOGATZEVSKA2
1 Sofia University “St. Kl. Ohridski”, Faculty of Biology, BG – 1164 Sofia, Bulgaria
2 Institute of Soil Science, Agrotechnologies and Plant Protection “N. Poushkarov”, BG – 1080 Sofia, Bulgaria

Abstract

Kizheva, Y., T. Vancheva, P. Hristova, M. Stoyanova, M. Stojanovska, P. Moncheva and N. Bogatzevska, 2013. Identification of Xanthomonas strains from tomato and pepper and their sensitivity to antibiotics and copper. Bulg. J. Agric. Sci., Supplement 2, 19: 80–82

This study aims to characterize 136 Xanthomonas strains causing bacterial spot of tomato and pepper from different regions in Bulgaria and Macedonia. Three specieswere identified by PCR with species-specific primers. Xanthomonas vesicatoria affects tomato and pepper and is the dominant species in Bulgaria on tomato while Xanthomonas euvesicatoria affects only pepper. This is the first report of Xanthomonas gardneri of tomato and X. euvesicatoria of pepper in Bulgaria. Amylase activity and ability to utilize cis-aconitate of the strains vary and cannot be used for species differentiation. The tested xanthomonads were sensitive to streptomycin. Copper ions in generally used concentrations in practice were effective only against pepper strains.

Key words: Xanthomonas vesicatoria, Xanthomonas euvesicatoria, Xanthomonas gardneri, bacterial spot, tomato, pepper

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