Antibiotic Complex, Produced by an Antarctic Actinomycete Strain S. Anulatus 39 LBG09

D. DIMITROVA1, P. DORKOV2 and B. GOCHEVA3
1 Medical University – Sofia, Medical College “Y. Filaretova “, BG – 0000 Sofia, Bulgaria
2 Biovet Ltd, R & D Directorate, BG – 0000 Peshtera, Bulgaria
3 Sofia University, Faculty of Biology, BG – 1164 Sofia, Bulgaria

Abstract

DIMITROVA, D., P. DORKOV and B. GOCHEVA, 2013. Antibiotic complex, produced by an Antarctic actinomycete strain S. Anulatus39 LBG09. Bulg. J. Agric. Sci., Supplement 2, 19: 72–76

 

The strain Streptomyces anulatus39 LBG09 was selected after screening of 17 actinomycete strains, isolated from samples of soil collected from Livingston Island, Antarctica, for production of antimicrobial substances. The strain synthesizes extracellular (86%) broad-spectrum antibiotic complex active against Gram-positive and Gram-negative bacteria including phytopathogenic bacteria. After discarding the mycelium the filtrates were extracted with several organic solvents for isolation and determination of polarity of the active substances. The most appropriate solvent was etylacetate, followed by acetone and diethyl ether. The complex mixture of several antibiotic substances with different polarity was found. The active extracts were subjected to TLC, additional purification through column chromatographic separation and HPLC-analysis. It was proved that the antibiotic complex consisted of three components: С1 (32.21%), С2 (13.21%) and С3 (18.33%). The components with polyene structure, as well as with spiranolactone and macrolactone rings were not found. The presence of aromatic rings was suggested. The antibiotic complex, synthesized by S. anulatus39 LBG09, was thermo- and alkali- stable.

Key words: antibiotic complex, component composition, screening, spectrum of activity
Abbreviations: Beer agar (BA), High Performance Liquid Cromatography (HPLC), Nutrient agar (NA), Minimal Inhibitory Concentration (MIC), Thin-Layer Cromatography Analysis (TLC)

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