Study Of Proteases And Protease Inhibitors From Streptomyces Strains

K. KALCHEV1, Y. RABADJIEV3, D. GANCHEV3, M. TSENOVA3, I. ILIEV2 and I. IVANOVA3
1 Shumen University, Department of Biology, BG – 9700 Shumen, Bulgaria
2 Plovdiv University “P. Hilendarski”, BG – 4000 Plovdiv, Bulgaria
3 Sofia University “St. K. Ohridski”, Faculty of Biology, BG – 1164 Sofia, Bulgaria

Abstract

Kalchev, K., Y. Rabadjiev, D. Ganchev, M. Tsenova, I. Iliev and I. Ivanova, 2013. Study of proteases and protease inhibitors from Streptomyces strains. Bulg. J. Agric. Sci., Supplement 2, 19: 65–67

 

Proteases and protease inhibitors are important enzymes in Streptomyces physiology and differentiation. The objective of this paper was to investigate the optimal conditions for production of proteases and protease inhibitors secreted by three newly isolated Streptomyces strains. The optimal growth and inhibition activities were detected after cultivation on different media and the maximum of protease inhibition activities were specific for the examined strains. The extracellular proteins were purified by ammonium sulfate precipitation and SDS-PAGE. One of the examined strains showed protease inhibitory activity perspective for future research.

Key words: Streptomyces, protease, protease inhibitors
Abbreviations: BAPNA – Nа-benzoyl-DL-arginine-ρ-nitro anilide, p-NA – p-nitro anilide

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