Effect of 6-Benzylaminopurine on Micropropagation of Artemisia Chamaemelifolia Vill. (Asteraceae)

L. HRISTOVA, E. DAMYANOVA, Z. DOICHINOVA and V. KAPCHINA-TOTEVA

Sofia University “St. Kliment Ohridski”, Department of Plant Physiology, Faculty of Biology, BG – 1164 Sofia, Bulgaria

Abstract

Hristova, L., E. Damyanova, Z. Doichinova and V. Kapchina-Toteva, 2013. Effect of 6-benzylaminopurine on micropropagation of Artemisia chamaemelifolia Vill. (Asteraceae). Bulg. J. Agri. Sci., Supplement 2, 19: 57–60

 

The influence of different concentrations (0.1–1.0 mg.L–1) of the cytokinin 6-benzylaminopurine (BA) on the in vitro propagation of the endangered aromatic plant Artemisia chamaemelifolia Vill. (Asteraceae) was examined. The increase of concentration stimulated the formation of shoots but shortened their length. The middle concentrations of 0.5, 0.6 and 0.7 mg.L–1 BA gave the highest number of shoots but they also appeared to promote negative effects like vitrification and necrosis more than the other variants. Although there was no clear trend, all variants had higher fresh weight and formed much more primary callus than the control explants grown on Murashige & Skoog medium with Gambourg vitamins. Over 50 % of the explants gave rise to more than 10 new shoots per explant at concentrations of 0.2, 0.3 and 0.9 mg.L–1 BA. All concentrations of BA suppressed root formation and manifested different extent of vitrification. This is the first report on the influence of BA on the micropropagation and growth of Artemisia chamaemelifolia Vill. The results obtained are standpoint for further biotechnological application for biomass and valuable metabolite production.

Key words: in vitro propagation, endangered species, cytokinin
Abbreviations: FW – fresh weight; DW – dry weight; BA – 6-benzylaminopurine or benzyl adenine

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