Callus Cultures and Indirect Regeneration of Ruscus Hypoglossum in vitro

T. IVANOVA, D. DIMITROVA, G. ANGELOV, Ch. GUSSEV, Y. BOSSEVA and T. STOEVA
Bulgarian Academy of Sciences, Department of Plant and Fungal Diversity and Resources, Institute of Biodiversity and Ecosystem, BG – 1113 Sofia, Bulgaria

Abstract

IVANOVA, T., D. DIMITROVA, G. ANGELOV, Ch. GUSSEV, Y. BOSSEVA and T. STOEVA, 2013. Callus cultures and indirect regeneration of Ruscus hypoglossum in vitro. Bulg. J. Agric. Sci., Supplement 2, 19: 49–51

 

Ruscus hypoglossum L. is a highly valued ornamental plant collected mainly from the wild and threatened in several European countries. Slow growth and low germination rates hamper its cultivation. Micropropagation has been considered advantageous for rapid production of planting material and ex situ conservation of Ruscus species. Callus cultures of R. hypoglossum were induced on TDZ containing medium and indirect shoot regeneration rate was evaluated on different medium composition. TDZ ensured higher regeneration rates, caused shoot, and cladode alterations in regenerates persisting throughout the cultivation. Callus induction was optimal at 30 g.l–1 sucrose and dropped down with the increase of sucrose concentration. Increase of the sucrose (15–60 g.l–1 ) influenced positively shoot proliferation. DNA content of the morphologically altered plants was not significantly different. Isozyme profile patterns demonstrated loss of isoform bands and/or induction of new ones.

Key words: genome size, isoenzymes, micropropagation, rhizome, Ruscaceae
Abbreviations: 6-benzylaminopurine (BAP), α-naphtaleneacetic acid (NAA), acid phosphatase (AP), esterase (EST), growth index (GI), kinetine (KIN), polyacrilamide gel electrophoresis (PAGE), peroxidase (POD), polyacrilamide gel electrophoresis (PAGE), thidiazuron (TDZ)

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