Effect of Chilling and Freezing Stresses on Jasmonate Content in Arabis Alpina

M. Kolaksazov1, F. Laporte2, K. Ananieva3, P. Dobrev4, M. Herzog2 and E. D. Ananiev1
1 University of Sofia, Faculty of Biology, Dept. Plant Physiology, BG – 1000 Sofia, Bulgaria
2 University of Grenoble Joseph Fourier, Laboratoire d’Ecologie Alpine – LECA
3 Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, BG - 1113 Sofia, Bulgaria
4 Institute of Experimental Botany, Prague, Czech Republic

Abstract

Kolaksazov, M., F. Laporte, K. Ananieva, P. Dobrev, M. Herzog and E. D. Ananiev, 2013. Effect of chilling and freezing stresses on jasmonate content in Arabis alpina. Bulg. J. Agric. Sci., Supplement 2, 19: 15–17

 

Depending on the temperature involved, there are two types of cold stress: chilling stress exerted at low positive temperatures around +5°C and freezing stress when temperatures are below zero. As an adaptation to cold stress, plants have evolved multiple mechanisms for tolerance. These mechanisms can be mediated by a number of stress phytohormones and plant growth regulators, including ABA, JA, SA, ethylene. It is well known that stress phytohormones exert their action by triggering phosphoprotein cascade pathways, which in turn lead to expression of genes, involved in the acquisition of cold stress tolerance. The perennial plant Arabis alpina (Brassicaceae), closely related to Arabidopsis sp. was selected as a model plant in this study. A. alpina is wide spread in mountain areas of the northern hemisphere, some of its populations being tolerant to frost. Using HPLC-MS analysis, we represent experimental data on JA and JA-ile content in three A. alpina populations exposed to chilling or freezing stress: frost tolerant (T), non-tolerant (NT) and plants with a short hypocotyl (SH).

Key words: Arabis alpina, cold stress (chilling, freezing), jasmonic acid, jasmonate-isoleucine
Abbreviations: ABA – abscisic acid, JA – jasmonic acid, JA-ile – jasmonate isoleucine, SA – salicylic acid, T – tolerant plants, NT – non-tolerant plants, SH – plants with short hypocotyl, LOX – lipoxygenase, DW – dry weight, HPLC – high pressure liquid chromatography

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