Fatty Acid Composition of Black Sea Ulva rigida and Cystoseira crinita

V. Ivanova1, M. Stancheva1 and D. Petrova2
1 Medical University, Department of Chemistry, BG – 9002 Varna, Bulgaria
2 Institute of Fish Resources (IFR), BG – 9000 Varna, Bulgaria


Ivanova, V., M. Stancheva and D. Petrova, 2013. Fatty acid composition of Black Sea Ulva rigida and Cystoseira crinita. Bulg. J. Agric. Sci., Supplement 1: 42–47


Green alga Ulva rigida and brown alga Cystoseira crinita are widespread in the Black Sea. There is limited information about lipid content and fatty acid composition of these species from Bulgarian Black Sea coast. The aim of this study was to determine and compare total lipid and fatty acid (FA) composition of Ulva rigida and Cystoseira crinita. Lipids were extracted by following the method of Bligh and Dyer. Fatty acid composition was analyzed by Gas Chromatography with MS detector. Total lipid content ranged between 0.72 and 0.79 g.100g-1 fresh weight. Generally, saturated fatty acids were major components (65–70%), with palmitic acid (C16:0) as the most abundant saturate (56–63%). Total polyunsaturated fatty acids (PUFAs) and monounsaturated fatty acids (MUFAs) ranged from 29% to 35%. The green alga showed higher C18 PUFAs contents than did C20 PUFAs. Cystoseira crinita belonging to the group of brown algae showed similar amounts of C18 and C20 PUFAs contents. The green alga was rich in linoleic acid (LA, C18:2n6) while the brown alga was rich in both linoleic acid (LA, C18:2n6) and eicosopentaenoic acid (EPA, C20:5n3). PUFA/SFA ratio in both species was approximately 0.35. All of the studied species had a nutritionally beneficial n6/n3 ratio (1.01–2.43:1).

Key words: Black sea algae, fatty acids, GC/MS
Abbreviations: FA – fatty acids; SFA – saturated fatty acids; MUFA – monounsaturated fatty acids; PUFA – polyunsaturated fatty acids; FAME – fatty acid methyl esters; GC – gas chromatography; MS – mass spectrometry

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