V. Nikolova and V. Todorova
Maritsa Vegetable Crops Research Institute, BG - 4003 Plovdiv, Bulgaria
NIKOLOVA, V. and V. TODOROVA, 2012. Possibility to select heterozygous genotypes by pollen fertility in segregation hybrid and backcross progenies created based on nuclear male sterility. Bulg. J. Agric. Sci., 18: 565-570
A successful attempt for separation of heterozygous (Msms) and dominant homozygous (MsMs) fertile genotypes based on pollen fertility in the segregating pepper backcross progenies with gene male sterile (ms8ms8) female parent was made. The study was done during 2007 – 2010 at the Maritsa Vegetable Crops Research Institute, Plovdiv, Bulgaria. The selected Msmsplants were included in pollination with the new male sterile forms to confirm their heterozygosis, to receive the segregation 1:1 male sterile (msms): male fertile (Msms) plants and to define fertile analogues. As a criteria in the determination of the fertile analogous plants – the pollinator with pollen fertility below 40% shows correct ratio near to the expected theoretical one 1:1 in maintenance of new male sterile forms. This method is faster and easier choice of fertile analogous and could be used in other plant species for selection of heterozygote genotypes by pollen fertility in segregation hybrid and backcross progenies based on nuclear male sterility.