Mutant Sunflower Line R 171, Produced through in vitro Mutagenesis of Immature Embryos

Dobroudja Agricultural Institute, BG – 9520 General Toshevo, Bulgaria


ENCHEVA, J., D. VALKOVA and P. SHINDROVA, 2012. Mutant sunflower line R 171, produced through in vitro mutagenesis of immature embryos. Bulg. J. Agric. Sci., 18: 342-347

Immature sunflower (Helianthus annuus L.) zygotic embryos of sunflower fertility restorer line 2571 R were treated with ultrasound before plating in the embryo culture medium. Some mutant plants were isolated and self-pollinated for several generations. New sunflower forms with inherited morphological and biochemical changes were obtained through selection and self-pollination. The genetic variations included 16 morphological and biochemical agronomic traits. In our study the plant height, length of branches, leaf petiole length, head diameter and seed yield per head were the most unstable, based on all investigated characters. In comparison to the control line 2571 R, increasing in the mean value of the indices was registered for 68.8 % of the total number of characters. Stability after the number of leaves, number of branches demonstrated induced mutagenesis, seed width and seed thickness. Reduction of mean value was register for 1000 seed weight. i.e. 6.3 % of the indices. Increasing of seed yield per head, oil content in seed and resistance to parasite Orobanche cumana of the new mutant line 171 RM is desire combination at breeding program of sunflower.

Key words: Helianthus annuus, embryo rescue, ultrasound, mutant line, resistance (Orobanche cumana)

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