Intestinal Microflora of Kutum Rutilus frisii kutum under Dietary Supplementation with Probiotic and Vitamin C

M. R. GHOMI1, Z. HESHMATIPOUR2, R. M. NAZARI3, M. SOHRABNEJAD4, M. ZAREI3, M. NIKOO5, M. OVISSIPOUR6 and A. ESMAEILI MOLLA3

1 Department of Fisheries Sciences, and Young Research Club, Islamic Azad University of Tonekabon, 46817, Tonekabon, Iran
2 Department of Microbiology, Islamic Azad University of Tonekabon, 46817, Tonekabon, Iran
3 Rajaei Sturgeon Fish Farm, P. O. Box 833, Sari, Iran
4 Department of Fisheries Sciences, Tarbiat Modarres University, Noor, Iran
5 Department of Fisheries, Tehran University, Karaj, Iran
6 Department of Fisheries, Gorgan Agricultural Sciences and Natural Resources University, Iran and Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway

Abstract

GHOMI, M. R., Z. HESHMATIPOUR, R. M. NAZARI, M. SOHRABNEJAD, M. ZAREI, M. NIKOO, M. OVISSIPOUR and A. ESMAEILI MOLLA, 2010. Intestinal microflora of kutum Rutilus frisii kutum under dietary supplementation with probiotic and vitamin C. Bulg. J. Agric. Sci., 16: 635-642

The purpose of this study was to evaluate the effect of dietary probiotic and vitamin C on intestinal microflora of kutum Rutilus frisii kutum juveniles. The juveniles were fed to apparent satiation the diet supplemented with probiotic Primalac® (0, 0.1 and 0.3%), vitamin C (0, 200 and 400 mg kg-1) or their combination for 8 weeks. Result indicated that fish fed with the control diets without supplementation exhibited statistically (P<0.05) higher mortality than other treatments. On the basis of carbohydrate utilization profiles using API 50 CHL kits (fermentation pattern), the intestinal detected strains were divided into the three groups (Lactobacillus brevis, L. plantarum and L. lactis). With increasing the culture period, number of total bacterial counts and lactic acid bacteria increased.

Key words: Probiotic, Vitamin C, Intestinal microflora, Kutum, Rutilus frisii kutum

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