Dehydrogenase Isoenzyme Polymorphism in Selected Almond Genotypes (Prunus amygdalus Batsch.)

S. COLICH1, D. MILATOVICH2, D. NIKOLICH2 and G. ZEC2
1 Institute for Science Application in Agriculture, 11000 Belgrade, Serbia
2 Institute for Fruit Science and Viticulture, Faculty of Agriculture, University of Belgrade, 11080 Belgrade, Serbia

Abstract

COLICH, S., D. MILATOVICH, D. NIKOLICH and G. ZEC, 2009. Dehydrogenase isoenzyme polymorphism in selected almond genotypes (Prunus amygdalus Batsch.). Bulg. J. Agric. Sci., 15: 552-556

Isoenzyme polymorphism was studied in 20 almond (Prunus amygdalus Batsch.) genotypes, selected in the region of northern Serbia (Vojvodina). Five enzyme systems: alcohol dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH) and shikimate dehydrogenase (SDH) were studied using the method of polyacrilamide gel electrophoresis. Three systems (FDH, GDH and SDH) were found to be polymorphic. This polymorphism allowed unique identification of five genotypes, while the remaining 15 genotypes were divided into five groups. Cluster analysis (UPGA method) was used to construct a dendrogram on which two clusters with different number of genotypes could be separated. Obtained results indicate that isoenzyme polymorphism in almond is higher than previously found. Studied enzyme systems, FDH, GDH and SDH, can be used to identify genetic variability in almond.

Key words: Prunus amygdalus, electrophoresis, dehydrogenase, polymorphism, cluster analysis
Abbreviations: aspartate aminotransferase (AAT), aconitase (ACO), acid phosphatase (ACP), alcohol dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), glucose phosphate isomerase (GPI), isocitrate dehydrogenase (IDH), leucine aminopeptidase (LAP), malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (PGD), phosphoglucomutase (PGM), shikimate dehydrogenase (SDH), polyacrilamide gel electrophoresis (PAGE)

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