Creating Sunflower Mutant Lines (Helianthus Annuus L.) Using Induced Mutagenesis

Dobroudja Agricultural Institute, BG-9520 General Toshevo, Bulgaria


ENCHEVA, J., 2009. Creating sunflower mutant lines (Helianthus annuus L.) using induced mutagenesis. Bulg. J. Agric. Sci., 15: 109-118

Immature sunflower zygotic embryos of sunflower fertility restorer line 374 R were treated with ultrasound and gamma radiation before plating embryos to culture medium. All plants were isolated and self-pollinated for several generations. New sunflower forms with inherited morphological and biochemical changes were obtained. The genetic changes occurring during the mutation procedure included fourteen morphological and biochemical characters.
In comparison to the check line 374 R, decreasing of the mean value of the indexes was registered for 33 % of the total number of characters and vise verse, significant increasing was observed for 60 %.
Mutation for resistance to the local population of Orobanche cumana (race A-E) was obtained from the susceptible Bulgarian control line 374 R. Two investigated mutant lines possessed 100 % resistance to Orobanche and stable inheritance in the next generations. Our results showed that induced mutagenesis in sunflower can be successfully used to develop new lines useful for heterosis breeding.

Key words: sunflower, Helianthus annuus, immature zygotic embryos, gamma radiation -137Cs, ultrasound, mutagenesis, new breeding material, resistance, Orobanche cumana

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