Study on Metabolic Implication of Supplemental Vitamins B3 + B6 in Exposed to Stress Chickens

P. MONEVA, S. POPOVA-RALCHEVA, D. GUDEV, V. SREDKOVA and I. YANCHEV
Institute of Animal Science, BG – 2232 Kostinbrod, Bulgaria

Abstract

MONEVA, P., S. POPOVA-RALCHEVA, D. GUDEV, V. SREDKOVA and I. YANCHEV, 2008. Study on the metabolic implication of supplemental vitamins B3 + B6 in exposed to stress chickens. Bulg. J. Agric. Sci., 14: 480-490

This study was undertaken to investigate the effect of supplemental vitamin B3 (40 mg.kg-1 diet) and vitamin B6 (7 mg. kg-1 diet) on some indices of stress in chickens under stress, induced by alternating periods of normal feeding with periods of feed and water withdrawal. Twenty chickens at the age of six weeks were randomly allocated into two groups- control and experimental. Experimental birds were deprived of feed and water and their legs tied for seventeen hours each day in four consecutive days. Blood samples were taken by 14h and 17h following the start of the last stressor episode. Supplemental vitamins B3 + B6 alleviated body weight decline, decreased spleen (P < 0.05) and liver (P < 0.001) relative weights, increased relative weights of adrenal glands (P < 0.05) but had no significant effect on that of bursa of Fabricius. Plasma urea levels were not influenced by supplemental vitamins B3+B6 but plasma glucose levels declined by 17h (P < 0.05) in control group. Cholesterol levels (P<0.05) was significantly lower by 17h in experimental group relative to control group. Vitamins B3+B6 supplementation did not prevent leukocyte numbers and H:L ratio increment caused by stressor treatment. Hematocrit values were significantly higher (P<0.01) by 14h in experimental birds in comparison with control chickens and baseline levels. Interleukin-1á response to stress declined in vitamins B3 + B6 supplemented broilers by 14 h and 17h while that of control group declined by 17h only. Corticosterone response to the applied stress stimuli was not influenced by supplemental B3 + B6. Our results indicate that vitamins B3+B6 are implicated in white blood cells dynamics and influence the pattern of the observed stress indices in chickens.

Key words: vitamins, B3, B6, stress, H:L ratio, IL-1á, white blood cells, corticosterone, chickens

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