Antioxidative Enzymes in Fish as Biochemical Indicators of Aquatic Pollution

L. VELKOVA-JORDANOSKA1, G. KOSTOSKI1 and B. JORDANOSKA
1 SI Hydrobiological Institute, Naum Ohridski, 50, 6000 Ohrid, R. Macedonia

Abstract

VELKOVA-JORDANOSKA, L., G. KOSTOSKI and B. JORDANOSKA, 2008.Antioxidative enzymes in fish as biochemical indicators of aquatic pollution. Bulg. J. Agric. Sci., 14: 235-237

Antioxidant enzyme activities of fish (Barbus m. petenyi Heck.) were determined to establish possible environmental impact of toxic effect on anthropogenic pollution on Lake Ohrid. Activities of superoxide dismutase (SOD) and catalase (CAT) in the blood of fish were chosen as bioindicators. Fish were sampled during May 2007 from locality Kalista (Lake Ohrid). After sacrificing the fishes freshly blood samples were collected and transported on ice. Superoxide dismutase (SOD) activity was measured by the ferricytochrome c method using xantine/xantine oxidase as a source of superoxide radicals. Catalase (CAT) activity was determinated by measuring the decrease of hydrogen peroxide concentration at 240 nm. Fish have been proposed as indicators for monitoring land-based pollution because they may concentrate indicative pollutants in their tissue, directly from water through respiration and also through their diet. Fish are frequently subjected to prooxidant effects of different pollutants often present in the aquatic environment.

Key words: B.m petenyi Heck., antioxidant enzymes, SOD, CAT, pollution

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