Distribution of Potato Leafroll Virus - (PLRV) and Potato Virus Y - (Pvyn) in a Field Experiment

A. KOTZAMPIGIKIS1, D.HRISTOVA2 and E. TASHEVA-TERZIEVA3
1Agricultural University, BG - 4000 Plovdiv, Bulgaria
2Plant Protection Institute, BG - 2232 Kostinbrod, Bulgaria
3 University of Sofia, Faculty of Biology, BG - 1000 Sofia, Bulgaria

Abstract

KOTZAMPIGIKIS, A., D. HRISTOVA and E. TASHEVA-TERZIEVA, 2008. Distribution of Potato Leafroll Virus - (PLRV) and Potato Virus Y - (Pvyn) in a field experiment. Bulg. J. Agric. Sci., 14: 56-67

The aphid activity flight and the distribution of PLRV and PVYn were studied in an experimental potato field.
The field experiment was carried out with three varieties of potatoes: Concorde - early, Arinda - medium early, Desiree - late, in an artificially created infectious background of 5% for each virus. Monitoring was carried out to identify viruliferous aphids through a biological test, for PLRV ELISA was also performed. Infection of potato plants was determined using DAS-ELISA.
The following aphid species vectors of both viruses were present: Myzus persicae Sulzer, Aphis gossypii Glover, Aphis fabae Scop., Aphis nasturtii Kalt., Aulacorthum solanii Kalt., Macrosiphum euphorbiae Thomas. It was monitored that M. persicae is a dominating vector with a peak in flight during the 27th week (4 - 10 July). A week after that, it was observed the highest percentage of plants infected with PVY n. The highest number of plants infected with PLRV was observed during the 30th week (25-31 July), three weeks after the peak in flight of aphids and a week after the observation of the highest number of wingless individuals.
PLRV causes a three-fold decrease in yield from the late and medium early variety. PVYn causes most severe damages to the early variety, reducing the yield four times. Mixed viral infection has the highest impact on yield with decrease in yield ranging from 4 to 5-fold, depending on the variety.

Key words: PLRV, PVY, dynamics of spread of infection, aphids as virus vectors, potatoes, yield

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