Thidiazuron-induced Regeneration in Root Segments of White Poplar (P. alba L.)
I. TSVETKOV1, J-F. HAUSMAN2 and L. JOUVE2
1Forest Research Institute, BG - 1756 Sofia, Bulgaria
2CRP Gabriel Lippmann, CREBS, 41, Department of Environment and Agrobiotechnology, Rue du Brill, L- 422 BELVAUX, Luxembour
TSVETKOV I., J-F. HAUSMAN and L. JOUVE, 2007. Thidiazuron-induced regeneration in root segments of white poplar (P. alba L.). Bulg. J. Agric. Sci., 13: 623-626
Root segments isolated from stably proliferating in vitro clone of white poplar (P. alba L.) were cultivated on modified MS medium supplemented with different thidiazuron concentrations. The thidiazuron concentration significantly affected the regenerative capacity of the explants. The lower doses firmly stimulated the regenerative ability of the root segments, while the stronger ones demonstrated inhibitive organogenic effect. The highest number of regenerated shoots was formed on medium supplemented with 0.56 µM thidiazuron, but longest shoots were produced at 0.02 µM thidiazuron.