Effects of Some Factors on the Development and Maturation of Common Oak (Q. robur L.) Somatic Embryos

I. TSVETKOV
Forest Research Institute, BG - 1756 Sofia, Bulgaria

Abstract

TSVETKOV, I., 2006. Effects of some factors on the development and maturation of common oak (Q. robur L.) somatic embryos. Bulg. J. Agric. Sci.,12: 761-765

The effects of some factors on the development and maturation of common oak (Q. robur L.) somatic embryos have been studied. Significant effects of both genotype and ABA exposure time on development and maturation of common oak somatic embryos were registered. The number of developed cotyledonary-stage somatic embryos was positively affected by the extension of the ABA exposure time, with relationship following a strong linear trend. Both the carbohydrate type and ABA dose significantly influenced the number of the cotyledonary-stage somatic embryos formed. The sucrose demonstrated sufficiently greater ability compared with maltose in promotion of the maturation of the somatic embryos. While no significant effect of the carbohydrate concentration was recognized, the lower ABA dose (0.38 μM) favoured in greater extent the process of somatic embryos development and maturation.

Key words: Abscisic acid, carbon source, in vitro, Quercus sp., somatic embryogenesis
Abbreviations: ABA - abscisic acid (+ ) cis-trans isomer, ET - embryogenic tissue, MS - Murashihe-Skoog nutrient medium