Zh. NANOVA1 and Y. SLAVOVA2
1 Episcope Constantine Preslavski University of Shumen,, BG - 9700 Shumen, Bulgaria
2 Agricultural Institute, BG - 9700 Shumen, Bulgaria
NANOVA, Zh. and Y. SLAVOVA, 2006. Mass vegetative propagation of winter marjoram. (Origanum vulgare ssp. hirtum (Link) Jetswaart). Bulg. J. Agric. Sci., 12: 531-536
It was conducted an experiment with introduction of winter marjoram (Origanum vulgare subsp. hirtum} in vitro culture and its micropropagation. The stem tips of the active growing under controlled conditions plant and young, one-week old, non-sterile germs of its seeds were used as primary explants. There were chosen suitable nutritional environments for growing, development and rooting. The optimal rooting effect (89.6 %) was reached under influence of 0.15 mg/1 IAA auxin (indolyl acetic acid) in nutritional environment. The green trimmings, taken from in vitro microreproducted plants after their one-month in vivo adapting, were rooted in a soil substrate consisting of 1:4 humustim: mixture of 1:1 sand and pearlite. It was observed rooting of above 90 % after 26-day cultivation under controlled conditions.