Optimization of Media for Rooting of Fodder Beet by Using Silver Nitrate (AgNO3)

Y. SLAVOVA, E. ZAYOVA and M. KASCHIEVA
Agricultural Institute, BG-9700 Shumen, Bulgaria

Abstract

SLAVOVA, Y., E. ZAYOVA and M. KASCHIEVA, 2006. Optimization of media for rooting of fodder beet by using silver nitrate (AgNO3). Bulg. J. Agric. Sci., 12: 515-519

The influence of AgNO3 (silver nitrate) on rooting of fodder beet in vitro was studied. Cloned plants obtained from isolated unpollinated ovules from 7 initial genotypes were used. Considerable limitation of late bacterial contamination accompanying in vitro cultivation of fodder beet was achieved by using AgNO3 (silver nitrate) in concentration from 1 to 20 mg/1 in rooting medium. It was observed 47.1±4.3 % rooting and 8.1% visible bacterial contamination under the influence of a medium without AgNO3 (control medium). The optimization of this medium was realized by using the following concentrations -1,2,3 and 4 mg/1 AgNO3 in it. Rooting changed immediately after using 1 mg/1 AgNO3 (68.3±3.9) and for the separate genotypes reached even 100%, but did not change considerably by using 2, 3, 4 and 5 mg/1 respectively. The higher concentrations of AgNO3 are recommendable only at presence of more persistent bacterial contamination.

Key words: fodder beet, in vitro, rooting, silver nitrate, bacterial contamination