Mapping of the Porcine CCS and CCR7 Genes

S. METODIEV1, B. MOTE2 and M. F. ROTHSCHILD2
1 Department Genetics and Animal Breeding, Agricultural Faculty, Thracian University, BG-6000 Stara Zagora, Bulgaria
2 Department of Animal Science, Center for Integrated Animal Genomics, Iowa State University, Ames, IA 50011 USA

Abstract

METODIEV, S., B. MOTE and M. F. ROTHSCHILD, 2006. Mapping of the porcine CCS and CCR7 genes. Bulg. J. Agric. Sci., 12: 153-157

CCS (cooper chaperon for superoxide dismutasel - SOD1) and CCR7 (chemokine (C-C motif) receptor 7 were studied as potential candidate genes associated with sow stress and were subsequently linkage mapped in the swine genome. Primers were designed for each gene from the pig CCS and CCR7 sequences found at the National Center for Biological Information. The CCS primer pair amplifies a 160 bp amplicon, while the CCR7 primer pair amplifies a 3 85bp amplicon. Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) tests with Nci I (New England Biolabs) for CCS and with HpyCh.4 III (New England Biolabs) for CCR7 were developed for genotyping polymorphisms. Pig CCS was mapped with strong linkage to SSC 2 markers SW2623, SWC9, SW2443, and SW2445 (LOD > 10). Pig CCR7 was mapped with strong linkage to SSC 12 markers SW874, S0090, S0147, and S0229 (LOD > 9). The present study of mapping the pig CCS and CCR7 genes increases information for SSC 2 and SSC 12 and it is a base to study CCS and CCR7 genes as candidate genes for sow stress and longevity.

Key words: CCS, CCR7, pigs, markers, linkage analysis