Molecular Characterization of Bulgarian Livestock Genetic Resources.
I. Genetic Diversity in Bulgarian Grey Cattle as Revealed by Microsatellite Markers

A. TENEVA1, E. TODOROVSKA2, N. TYUFEKCHIEV3, L. KOZELOV1, A. Atanassov2, S. FOTEVA1, S. RALCHEVA1 and S. ZLATAREV4
1 Institute of Animal Sciences, BG-2232 Kostinbrod, Bulgaria
2 AgroBio Institute, BG-1164 Sofia, Bulgaria
3 Research Institute of Forensic Science and Criminology, BG -1000 Sofia, Bulgaria
4 Institute of Experimental Pathology and Parasitology, BG-1113 Sofia, Bulgaria

Abstract

TENEVA, A., E. TODOROVSKA, N. TYUFEKCHIEV, L. KOZELOV, A. ATANASSOV, S. FOTEVA, S. RALCHEVA and S. ZLATAREV, 2005. Molecular characterization of Bulgarian livestock genetic resources. I. Genetic diversity in Bulgarian Grey cattle as revealed by microsatellite markers. Bulg. J. Agric. Sci., 11: 363-372

Within _breed genetic diversity of a herd from indigenous Bulgarian Grey cattle breed was assessed for the first time with a microsatellite set (TGLA 227, BM 2113, TGLA 53, ETH 10, SPS 115, TGLA 126, TGLA 122, INRA 23, ETH 3, ETH 225, BM 1824) of the Internationally accepted panel for the cattle biodiversity study. Polymorphism in eleven SSR loci in a sample of 35 animals was evaluated and compared to other bovine populations.
In the 11 loci studied with an automated DNA sizing technology, 83 alleles were identified. Microsatellites were highly polymorphic with a mean number (MNA) of 7.6 alleles (ranging from 4 to 12 per locus). For each locus, allele frequencies, observed heterozygosity (Ho), gene diversity (He) and polymorphic information content (PIC) were computed. The average Ho (0.78), He (0.86) and PIC (0.72) displayed a high level of genetic variation. The present study confirmed the high polymorphism of the chosen set of microsatellite markers and its usefulness in determination of the population structure of bovine genetic resources from Bulgaria.

Key words: cattle, microsatellites, polymorphism, genetic resources