Influence of Some New Biologically Active Substances on in vitro Rooting of Medicinal Betony (Stachys officinalis Trev./ L.)

Y. SLAVOVA1, D. NENKOVA2 and I.IVANOVA2
1 Institute of Agriculture, BG-9700 Shumen, Bulgaria
2 Institute of Cryobiology and Food Technology, BG-1407 Sofia, Bulgaria

Abstract

SLAVOVA, Y., D. NENKOVA and I. IVANOVA, 2005. Influence of some new, biologically active substances on in vitro rooting of medicinal Betony (Stachys officinalis Trev./ L.). Bulg. J. Agric. Sci., 11: 135-139

The subject of the study was the influence of different concentrations of biologically active substances (BAS) - BI (Bezymidazol); A-250 (derivative of the butyric acid, microelements, vitamins); PP - 40 (derivative of the phenoxy acetic acid and the folic acid); Ryzomil (derivative of the naphtyl acetic acid) and PF - 32 (derivative of the phenoxy acetic acid, vitamins) within nutritive medium in vitro for rooting of Stachys officinalis. In vitro cloned plants were used as initial material. Three of the used substances showed very good rooting, and namely these: 0.5 mg/L, BI 82.9±4.6%; 1.0 mg/L A-250 - 80.3±4.6% and 0.5 mg/L PF-32-79.3±7.5%.
Along with the successful rooting, the concentrations of the cited substances stimulate the normal growth of the plants, delaysaging, which prolongs the passage and guarantees the successful in vitro adaptation.

Key words: Stachys officinalis, biologically active substances (BAS), in vitro rooting, nutritive medium