Study on a Doubled Haploid Line of Helianthus annuus L., Produced by Gamma-Induced Parthenogenesis

M. DRUMEVA1, A. BERVILLE2, P. IVANOV1, N. NENOVA1 and J. ENCHEVA1

1 Dobroudja Agricultural Institute, BG - 9520 General Toshevo, Bulgaria
2 INRA, UR de Genetique et d'Amelioration des Plantes, 2 Place Pierre Viala, 34060 Montpellier cedex 1, France

Abstract

DRUMEVA, M., A. BERVILLE, P. IVANOV, N. NENOVA and J. ENCHEVA, 2005. Study on a doubled haploid line of Helianthus annuus L., produced by gamma-induced parthenogenesis. Bulg. J. Agric. Sci., 11: 129-134

Molecular analysis was applied to the first selfed generation (P2) of line 99A65 developed by the method of gamma-induced parthenogenesis. The line was obtained from hybrid Albena with the participation of pollen source 1395 R, the pollen of which was treated with g-rays at dose 900 Gy.
Twelve microsatellite (SSR) primers were used to analyze the line. DNA was isolated from plant leaves at stage third-fourth pair. Samples were taken from five random individuals in P2 of the investigated line. Samples for DNA analysis were taken from the parental lines of hybrid Albena - line 2607 A and 147 R, as well as from the hybrid itself. Among the 12 SSR primers used, two-showed polymorphism in the analyzed plants - SSL51 and SSU63.
The analysis carried out revealed that pollen source 1395 R did not participate with own genetic material in the process of formation and development of the line 99A65, which was an indirect evidence for its doubled haploid origin. This was further confirmed by the homozygocity established in progeny P2 of the line in locus SSL51. In locus SSU63 homozygocity could not be established due to the lack of polymorphism between the parental lines of hybrid Albena.

Key words: gamma-induced parthenogenesis, sunflower (Helianthus annuus L.), SSR markers, doubled haploid