Effect of Carbohydrates on the Growth Dynamics of Sweet Cherry (Prunus avium L.) Callus Cultures

P. GERCHEVA, P. SHALAMANOVA and P. MANOLOV
Fruit Growing Institute, BG-4004 Plovdiv, Bulgaria

Abstract

GERCHEVA, P., P. SHALAMANOVA and P. MANOLOV, 2005. Effect of carbohydrates on the growth dynamics of sweet cherry (Prunus avium L.) callus cultures. Bulg. J. Agric. Sci., 11: 29-34

The available literature information and our preliminary studies showed that the type and concentration of carbohydrate sources affected strongly in vitro morphogenetic processes when standard concentrations of growth regulators were used.
The objective of the present work was to study the effect of the carbohydrate composition of media on the growth dynamics of sweet cherry callus cultures.
Callus of leaf tissues from in vitro cultured seedlings of the sweet cherry cultivar Bigarreau Burlat was used as source material. Sixteen nutrient media with permanent mineral and hormonal compositions (basic MS, TDZ 7.5 μM and IBA 2.5 μM) and varying carbohydrate components (sucrose, sorbitol and mannitol) were tested. The initial callus amount in all variants was 0.2g. The quantitative measurements were made under sterile conditions at seven-day intervals with 10 test tubes per variant for 42 days.
The results obtained justified our suggestion that sorbitol had metabolic rather than osmotic effect on the growth of sweet cherry callus in vitro. This effect was especially evident at high carbohydrate concentrations after 4 weeks of culture.

Key words: sweet cherry (Prunus avium), callus, sorbitol, mannitol, sucrose