J. ENCHEVA1, M. CHRISTOV1, H. KÖHLER2, P. SHINDROVA1, V. ENCHEVA1 and W. FRIEDT2
1 Dobroudja Agricultural Institute, BG-9520 General Toshevo, Bulgaria
2 Institute of Plant Growing and Plant Protection, Giesen, Germany
ENCHEVA, J., M. CHRISTOV, H. KÖHLER, P. SHINDROVA, V. ENCHEVA and W. FRIEDT, 2004. Interspecific hybrids between Helianthus annuus and H. tuberosus: RAPD analysis, disease resistance, combining ability. Bulg. J. Agric. Sci., 10: 169–175
The method of direct organogenesis has been successfully used for overcoming the inability for crossing. As a result from successful hybridization between Helianthus annuus (cv. Albena) and H. tuberosus lines R 101 and R 104 were developed. They possess very good combining ability in hybridization with the sterile analogue of the Bulgarian line 2607 with cytoplasm of Helianthus petiolaris. Line R 104 is also characterized with resistance to the local population of the parasite broomrape against up to 70 % artificial infection background The gene for resistance was transferred from species H. tuberosus, which, according to the studies of Pustovoit, 1978 and Christov, 1996 is completely resistant to the parasite. Line R 101 showed resistance and line R 104 tolerance to phoma. Resistance to downy mildew (race 300) demonstrated the both investigated lines.
The molecular RAPD analysis carried out on stable self-pollinated lines confirmed the hybrid nature of the breeding materials obtained from the cross between H. annuus and H. tuberosus.