Intergeneric Hybrids between Cultivated Sunflower (Helianthus annuus L.) and Verbesina helianthoides (Genus Verbesina) - RAPD Analysis

J. ENCHEVA1, H. KÖHLER2, M. CHRISTOV1 and W. FRIEDT2
1Dobroudja Agricultural Institute, BG - 9520 General Toshevo, Bulgaria
2Institute of Plant Growing and Plant Protection, Giesen, Germany

Abstract

ENCHEVA, J., H. KÖHLER, M. CHRISTOV and W. FRIEDT, 2004. Intergeneric hybrids between cultivated sunflower (Helianthus annuus L.) and Verbesina helianthoides (Genus Verbesina) - RAPD Analysis. Bulg. J. Agric. Sci., 10: 723-728

The method of direct organogenesis has been successfully used for overcoming the inability for crossing between Helianthus annuus (cv. Albena) and Verbesina helianthoides (genus Verbesina ). Therefore, from long-term individual selection in the hybrid materials, fertility restorer lines were produced in R 10 generation. The molecular method RAPD confirmed the hybrid nature of the obtained breeding material and indicated an introgression of Verbesina helianthoides DNA into some of the hybrid progenies produced. By using the similarity coefficient of Nei and Li, 1979 a dendogram was generated through UPGMA analysis with the aim to determine the genetic distance of the studied genotypes. We were able to demonstrate that RAPD could be used for characterization of intergeneric hybrid progenies in sunflower at a late stage of selection (F9) in which an increased genetic variation was discovered.

Key words: Helianthus annuus, Verbesina helianthoides (genus Verbesina), direct organogenesis, RAPD analysis