Study of the Influence of -1, 3, 4 Oxadiazine on the Growth, Morphology and the Macromolecular Synthesis of the Primary Cell Cultures

D. ILIEVA1, E. ILIEV2, A. GALABOV3, L. VASSILEVA3, Y. SERKEDJIEVA3 and G. GEORGIEV4
1 National Diagnostic Research Veterinary Institute, BG-1606 Sofia, Bulgaria
2 General Directorate of the National Veterinary Service, BG-1606 Sofia, Bulgaria
3 Institute of Microbiology - Bulgarian Academy of Sciences, BG-1113 Sofia, Bulgaria
4 Medical University, Faculty of Pharmacy, BG-1000 Sofia, Bulgaria

Abstract

ILIEVA, D., E. ILIEV, A. GALABOV, L. VASSILEVA, Y. SERKEDJIEVA and G. GEORGIEV, 2004. Study of the influence of bromine derivative of 2-/4 uracilmethylene/5-fenyl-6 hydroxy-2,3-dihydro/6H/-1,3,4 oxadiazine on the growth, morphology and the macromolecular synthesis of the primary cell cultures. Bulg. J. Agric. Sci., 10: 479-484

A bromine derivative of 2-/4 uracilmethylene/5-fenyl-6 hydroxy-2,3-dihydro/6H/-1,3,4 oxadiazine (BUFOD) was tested for toxicity on primary cell cultures of chicken embryonal fibroblasts (CEF) and to calf kidney (CK). The effect of BUFOD was traced on (a) cell growth; (b) cell morphology in the stationary stage of cell culture monolayer; (c) cell macromolecular synthesis (RNA, DNA and protein) by means of inclusion of radioactive marked precursors - method by using radioactivity measurement of acid nonsoluble fraction.
The maximum tolerance concentration of BUFOD when tracing the growth of the cell cultures (CC) and their effect on the monolayer cultures was 200 mg/cm3 for both CC (CEF and CK).
In contrast to these data, during investigation of BUFOD influence on the cell macromolecular synthesis the following results were obtained: the RNA synthesis in CEF was suppressed, at 24 h at a concentration of 100 mg/cm3 - 35.6 %, and at 200 mg/cm3 - 60.1 %. The synthesis of cellular DNA at 24 h, was inhibited - 45.2 % at 200 µg/cm3. The protein synthesis at a concentration of 200 µg/cm3 was influenced - 54.3 % at 24 h, respectively.

Key words: bromine derivative of 2-/4 uracilmethylene/5-fenyl-6 hydroxy-2,3-dihydro/6H/-1,3,4 oxadiazine, primary cell cultures, radioactive marked precursors