Element Content and Distribution in Cereal Crops Grown on Copper Polluted Soil
1. Effect of Excess Copper on Element Uptake in Barley

I. BOGOEVA and E. MITEVA
Plant Protection Institute, BG-2230 Kostinbrod, Bulgaria

Abstract

BOGOEVA, I. and E. MITEVA, 2003. Element content and distribution in cereal crops grown on copper polluted soil 1.Effect of excess copper on element uptake in barley. Bulg. J. Agric. Sci., 9: 305–311

Two cultivars of barley (Hordeum distichum and Hordeum vulgare) were grown in a greenhouse pot experiment. Copper was applied into the soil in various concentrations: 150, 250, and 400 mg.kg–1. An uncontaminated soil was used for control plants. Content of elements was determined in plant samples (roots, stems and seeds) in phenophase ripening. Atomic absorption spectrometry was used for determination of Cu and other elements: Fe, Mn, Mo, Na, K, Mg, Ca, Zn; the concentration of N was measured by classical Keldal`s method and of P – colorimetricaly.
The accumulation of Cu was increased with enhanced concentration of its application into the soil. A positive correlation between copper concentration in the soil and its uptake in roots of barley was also determined. The presence of other observed elements was influenced by increasing copper quantity. It was also established that winter barley accumulated higher copper concentration in roots, compared to spring barley. On the contrary – copper content in seeds of spring barley was higher than those, measured in winter barley, without exceeding the limit permitted concentration.

Key words: element uptake, heavy metal pollution, copper accumulation, Atomic absorption spectrometry, barley