Vegetative Propagation of Wood Betony (Stachys officinalis (Trev.) L.) Cultured in vitro

D. ZAHARIEV1 and Y. SLAVOVA2
1 University of Shumen "Episcop Konstantin Preslavsky", BG-9700 Shoumen, Bulgaria
2 Agricultural Institute, BG-9700 Shumen, Bulgaria

Abstract

ZAHARIEV, D. and Y. SLAVOVA, 2003. Vegetative propagation of Wood betony (Stachys officinalis (Trev.) L.) cultured in vitro. Bulg. J. Agric. Sci., 9: 197–202

The focus of our research is the possibility for adapting a vegetative propagation method, which has already been applied on sugar beet (Beta vulgaris L.), for the species of Wood betony (Stachys officinalis (Trev.) L.) The initial material (several-days-old seedlings) has been sterilized with 0.04% HgCl2. The micro propagation of the cultured plants has been carried out in growth medium by using 0.2 mg/l of BAP (6-benzilaminopurine), 0.2 mg/l GA3 (gibberellic acid) and 0.5 mg/l of TDZ (thidiazuron). The cloned plants has been rooted in two growth media by using 1.0 mg/l NAA (α-1-naphthylacetic acid) with 3% and 4% sucrose respectively. We came to the conclusion that the used growth media are apt for the investigated species. The plants grow and develop normally. Within a period of 20–25 days in growth medium for micro propagation have been developed between 2 and 9 new buds out of one vegetative one. The rooting itself takes place within 15–20 days, as the percentage of the plants rooted is about 70%. The cultured in vitro plants from the species Stachys officinalis continue to exist and grow successfully in vivo.

Key words: Stachys officinalis, cultured in vitro, growth medium, micro propagation, rooting