Study on Phytase-Producing Yeasts

S. GARGOVA, I. STOILOVA and G. VANCHEVA
University of Food Technologies, Department of Biotechnology, BG-4002 Plovdiv, Bulgaria

Abstract

GARGOVA, S., I. STOILOVA and G. VANCHEVA, 2003. Study on phytase-producing yeasts. Bulg. J. Agric. Sci., 9: 573–578

A two-stage screening-procedure was implemented involving phytase-producing yeasts, which resulted in the selection of Candida melibiosica 2491, possessing a high capacity for synthesis of an intracellular enzyme. The strain showed two maximums of phytase production – on the 8th h and on the 36th h of the growth in a nutrient medium containing glucose 10 g/dm3. Adding KH2PO4 and Na-phytate as supplementary sources of phosphorus heavily limited the biosyhtesis process.

Key words: yeasts, phytase, Candida melibiosica, production