Gliadin Analysis for Identification of 1BL/1RS Wheat-Rye Translocations and for Assessment of Protein Patterns in Aluminium Tolerant Genotypes

S. D. STOYANOVA, D. B. BOYADJIEVA and Ch. Ch. PHYLIPOV
Institute for Plant Introduction and Genetic Resources, BG-4122 Sadovo, Bulgaria

Abstract

STOYANOVA, S. D., D. B. BOYADJIEVA and Ch. Ch. PHYLIPOV, 2001. Gliadin analysis for identification of 1BL/1RS wheat-rye translocations and for assessment of protein patterns in aluminium tolerant genotypes. Bulg. J. Agric. Sci., 7: 255-260

Wheat germplasm of different origin (58 accessions) were evaluated by acid gliadin electrophoresis to describe genotypes possessing 1BL/1RS translocation. Wheat cv. Marquis was used to detect the relative mobility (RM) of gladin components. As a marked for 1RS group of bands in the area of w-gliadins was used wheat cv. Kavkaz. Aluminium tolerance was described both for 1BL/1RS translocated wheat and for non-translocated. The effect of aluminium was presented by root growth indexes at four Al-media (0 mM, 0.18 mM, 0.36 mM, 0.72 mM and 1.4 mM). Cluster analysis of gliadin components was carried out to specify the association between gliadin patterns and tolerance to toxic aluminium. 1BL/1RS translocated wheats were found to be more tolerant to Al-toxicity.

Key words: wheat, 1BL/1RS translocation, gliadin, electrophoresis, aluminium tolerance