Artificial Induction of Triploidy in Brook Trout (Salvelinus fontinalis): Attempt for Heat Shock Optimization
E. UZUNOVA1, A. MICHOVA2, H. TASKOV2 and M. PESHEVA1
1 Sofia University, Faculty of Biology, BG-1241 Sofia, Bulgaria
2 National Center for Infection and Parasitic Diseases, BG-1241 Sofia, Bulgaria
UZUNOVA, E., A. MICHOVA, H. TASKOVand M. PESHEVA, 2000. Artificial induction of triploidy in brook trout (Salvelinus fontinalis): attempt for heat shock optimization. Bulg. J. Agric. Sci., 6: 463-468
Heat shocks were applied on newly fertilized eggs to produce triploid brook trout by retention the second polar body. Ploidy analyses were carried out by flow cytometry technique. Multi-embryo homogenates from each experimental group was analyzed 5 to 10 days after treatment. Individual fry were analyzed as well. Both analyses revealed triploid induction ranging from 13 to 87% depending on the temperature and timing of the heat- shock treatment. Several reasons for low survival rates are discussed.
Key words: triploidy, heat-shock, brook trout