1BL/1RS Translocations in Wheat Germplasm in Relation to Aluminium Tolerance
S. D. STOYANOVA, D. B. BOYADJIEVA and Ch. Ch. PHYLIPOV
Institute for Introduction and Genetic Resources, BG – 4122 Sadovo, Bulgaria
STOYANOVA, S. D., D. B. BOYADJIEVA and Ch. Ch. PHYLIPOV, 2000. 1BL/1RS translocations in wheat germplasm in relation to aluminium tolerance. Bulg. J. Agric. Sci., 6: 155-159
64 wheat accessions were evaluated to detect the genotypes bearing 1B/1R wheat-rye chromosomal translocation Acid polyacryamide gel electrophoresis (A-PAGE) of gliadins was used to identify the presence or absence of 1BL/1RS translocation comparing w -gliadins in wheat cv. Kavkaz, which poses a typical Gli B3 locus. The tolerance to aluminium toxicity was evaluated both for 1B/1R traslocated wheat and for non-translocated. The effect of aluminium was presented by root growth indexes at four Al-media (0 mM, 0.18 mM, 0.36 mM and 1.4 mM). The results at this stage of investigations presented that 1B/1R translocated wheat genotypes could be suggested more tolerant to toxic aluminium.
Key words: wheat, 1B/1R translocation, gliadin, electrophoresis, aluminium tolerance