Effect of Dietary Composition and Phosphorus Level on Some Biochemical Parameters in Jejunal Enterocytes of Broiler Chicks

E. T. TONCHEVA and H. J. STANCHEV

Institute of Animal Breeding, BG-2232 Kostinbrod, Bulgaria

Abstract

TONCHEVA, E.T. and H. J. STANCHEV, 1999. Effect of dietary composition and phosphorus level on some biochemical parameters in jejunal enterocytes of broiler chicks. Bulg. J. Agric. Sci., 5: 927-932

Two 56-day trials were conducted (n = 20 and n 20) to evaluate the response of feeding corn-soybean meal diet and fish meal diet (basal diets) with equal content of total P (0.59%) and available P (0.29%) supplemented with 0.2, 0.3 and 0.4% Pin. (as dicalcium phosphate) on alkaline phosphatase activity (EC 3.1.3.1) in blood serum and jejunal enterocytes, Na+, K+ ATPase activity (EC 3.6.1.3) and P-RNA and P-DNA concentration in jejunal enterocytes of broiler chicks.
There was established a considerable increase in serum and enterocyte alkaline phosphatase activity of chicks fed basal diets compared to the enzyme activity after supplementation with 0.2% (to FMBD) 0.3% (to CSBMBD) Pin. The highest Na+, K+ ATPase activity was indicated in jejunal enterocytes of broilers fed basal diets supplemented with 0.2% (to CSBMBD) 0.3% (to FMBD) Pin. P-RNA concentration and protein:P-DNA ratio increased after supplementation of basal diets with 0.4% Pin.
The data suggest that biochemical consequences coming as a results of feeding phosphorus deficient basal diets were eliminated after supplementation with 0.2 0.3% Pin as dicalcium phosphate.

Key words: chick, dietary phosphorus, deficit, small intestine, serum, alkaline phosphatase, Na+, K+ ATPase, RNA, DNA