Establishment of Squash Mosaic Virus (SqMV)in Seeds of Sugarbeet and Cucurbit Crops by ELISA Method

B. DIKOVA

Plant Protection Institute, BG-2230 Kostinbrod, Bulgaria

Abstract

DIKOVA, B., 1999. Establishment of Squash Mosaic Virus (SqMV) in seeds of sugarbeet and cucurbit crops by ELISA method. Bulg. J. Agric. Sci., 5: 585-588

The glasshouse and laboratory experiments were carred out with plants and their seeds from sugarbeet crops (Beta vulgaris var. saccharifera) and cucurbit crops (Cucurbita spp.) during 1997-1998 at the Plant Protection Institute in Kostinbrod, Bulgaria.
The aim of the study was to establish seed transmission of Squash Mosaic Virus (SqMV) by the seeds of sugarbeet plants and cucurbit plants with blistered and deformed fruits. The sugarbeet leaves, the racemes and the germinated seeds from sugarbeet plants and the seeds from the cucurbit fruits were tested by enzime linked immunosorbent assay (ELISA), variant-indirect ELISA (I ELISA ) with antiserum to squash mosaic virus (SqMV) by Dr. Lisa, Itali and alkalinephosphatase antirabbit conjugate by SIGMA.
It was established that SqMV existed in leaves, racemes and seeds of sugarbeet plants. Squash Mosaic Virus (SqMV) was present in 7 and 14 of seeds, that was 35% and 56% of them. In Cucurbita pepo (summer squash) SqMV was contained in 45% of the germinated seeds, and in Cucurbita maxima (winter squash) only 11%. Since SqMV existed in germinated seeds of sugarbeet plants, summer and winter squash, it was therefore transmitted together with their seeds. As Lockhart et al. (1985) expected, the sugarbeet crops take part in the epidemiology of Squash Mosaic Comovirus (SqMV). In this paper the hipothesis for the existance of SqMV in sugarbeet plants and in their seeds was confirmed.

Key words: sugarbeet and cucurbit species, seed transmission, Squash Mosaic Comovirus (SqMV), indirect ELISA