Characterization of Cucumber Mosaic Virus (CMV) Tolerance in Cucumber Breeding Lines Using Indirect ELISA
D. KOSTOVA*, A. KARPAROV** and M. ALEXANDROVA*
* Maritsa Vegetable Crops Research Institute, BG-4003 Plovdiv, Bulgaria
** National Center of Infectious and Parasitic Diseases, BG-1000 Sofia, Bulgaria
KOSTOVA, D., A. KARPAROVand M. ALEXANDROVA, 1999. Characterization of Cucumber Mosaic Virus (CMV) tolerance in cucumber breeding lines using indirect ELISA. Bulg. J. Agric. Sci., 5: 571-575
Investigation has been carried out in order to distinguish different levels of tolerance in Cucumber Mosaic Virus (CMV) infected cucumber genotypes, derived from a cross between susceptible and tolerant parents. Indirect ELISA was used for a quantitative assessment of the virus concentration.
Based on the obtained results “CMV tolerance” should be considered as an ability to depress virus multiplication or an ability to depress symptom expression or both of them.
Indirect ELISA is an excellent assay for differentiation of highly susceptible genotypes from tolerant ones. But it is not sufficiently precise to distinguish negligible levels of CMV tolerance in segregating generations.
Key words: cucumber, CMV tolerance, breeding, indirect ELISA