Isolation of Mesophyll Protoplasts from Prunus domestica cv. Quetche

V. KONDAKOVA

Institute for Plant Protection, BG 2230 Kostinbrod, Bulgaria

Abstract

KONDAKOVA, V., 1999. Isolation of mesophyll protoplasts from Prunus domestica cv. Quetche. Bulg. J. Agric. Sci., 5: 411-414

An effective system for isolation of mesophyll protoplasts was developed. The effect of several enzyme mixtures was tested. An yield of 20.4 x106 protoplsts/g leaf tissue with viability 92% was obtained after digestion of tissue in enzyme solution containing cellulase "Onozuca" RS 1%, hemicellulase 1%, pectolyase Y-23 0.1% and 0.7M manitol as osmotic.
The plating of protoplsts in medium containing MS basal medium with NAA 0.01 mg/l, BAP 1.5 mg/l and zeatine 0.5 mg/l with osmotic as 0.5M sucrose had better influence on the beginning of first division and development of protoplasts.
Key words: Prunus domestica, mesophyll protoplasts, enzymes, osmotics